Pearson Edexcel As Biology B Paper 2 2025 Question Paper

Download the official Pearson Edexcel AS Level Biology B Paper 2 question paper from the June 2025 examination series (8BI0/02). This examiner-authentic PDF contains the complete Paper 2 assessment used in the live Edexcel AS Biology B examination. The paper covers Core Physiology and Ecology, testing biological knowledge, calculations, graph interpretation, experimental design, data analysis, and practical-investigation skills under real Edexcel exam conditions.

The paper includes questions on cell surface membranes, cholesterol, integral proteins, water channels, diffusion, sodium ion transport, arteries and veins, blood clotting, atherosclerosis, the cardiac cycle, fish circulation, allopatric and sympatric populations, cricket courtship behaviour, natural selection, haemoglobin oxygen dissociation curves, osmosis in potato cylinders, transpiration in alfalfa plants, and gas exchange systems in mammals and insects.

Students are required to analyse graphs, interpret scientific data, complete biological calculations, explain physiological and ecological processes, evaluate practical methods, and apply biological concepts to realistic scientific contexts. Practical and analytical questions include transpiration investigations, osmosis experiments, locust ventilation-rate studies, blood-clotting sequences, and ecological analysis involving speciation in crickets.

Further sections assess understanding of oxygen affinity in vicunas and llamas, environmental effects on transpiration rate, insect ventilation, mammalian gas exchange, cardiovascular physiology, fish circulatory systems, and the influence of temperature and humidity on water movement in plants. Students must apply scientific reasoning and accurate biological terminology throughout the assessment.

This 80-mark assessment is ideal for timed practice, classroom revision, mock examinations, independent study, and advanced AS Biology preparation before the 2026 examination series. Pair this paper with the matching Edexcel 8BI0/02 June 2025 mark scheme and examiner guidance on markscheme.net for complete examiner-standard revision and accurate self-assessment.

This document is designed for Pearson Edexcel AS Level Biology B students studying the 8BI0 specification, especially Year 12 students preparing for the 2026 examination series who want authentic practice using official Edexcel assessment materials. It is particularly valuable for students aiming for high grades who need experience with graph interpretation, biological calculations, practical-investigation design, experimental evaluation, ecological analysis, and extended scientific explanations under timed exam conditions.

The paper is also highly useful for Biology teachers, tutors, sixth-form colleges, revision centres, home-schooled learners, and retake candidates who require official Edexcel exam-standard resources for mock examinations, classroom assessments, practical-skills development, homework tasks, and structured revision programmes. It helps learners strengthen scientific reasoning, analytical skills, physiological understanding, ecological application, and exam technique before the 2026 AS Biology examinations.