Pearson Edexcel As Biology B Paper 1 2025 Question Paper

Download the official Pearson Edexcel AS Level Biology B Paper 1 question paper from the June 2025 examination series (8BI0/01). This examiner-authentic PDF contains the complete Paper 1 assessment used in the live Edexcel AS Biology B examination. The paper covers Core Cellular Biology and Microbiology, testing biological knowledge, practical understanding, graph interpretation, calculations, data analysis, and experimental-design skills under real Edexcel exam conditions.

The paper includes questions on trisaccharides, alpha glucose structure, glycosidic bonding, DNA semi-conservative replication, complementary base pairing, phospholipid bilayers, mitochondria, electron microscopy, intermembrane spaces, organelles with double membranes, stomata, guard cells, potassium ion transport, water surface tension, hydrogen bonding, mosquito adaptations, pollen grain germination, pollen tube growth, Q10 calculations, viruses, Ebola outbreaks, epidemiology, and end-product inhibition in metabolic pathways.

Students are required to analyse graphs, interpret scientific data, complete biological calculations, explain molecular and cellular processes, evaluate practical methods, and devise investigations involving stomatal pore width, potassium ion concentration, pollen grain germination, and viral disease control. The paper also includes practical and analytical questions involving microscopy, surface tension, temperature effects on enzymes, and epidemiological modelling.

Further sections assess understanding of mitochondria division, phospholipid membrane structure, pollen tube nuclei, viral transmission, climate and land-use effects on disease spread, enzyme specificity, activation energy, metabolic pathways, and feedback inhibition. Students must apply biological terminology and scientific reasoning to realistic biological contexts.

This 80-mark assessment is ideal for timed practice, classroom revision, mock examinations, independent study, and advanced AS Biology preparation before the 2026 examination series. Pair this paper with the matching Edexcel 8BI0/01 June 2025 mark scheme and examiner guidance on markscheme.net for complete examiner-standard revision and accurate self-assessment.

This document is designed for Pearson Edexcel AS Level Biology B students studying the 8BI0 specification, especially Year 12 students preparing for the 2026 examination series who want authentic practice using official Edexcel assessment materials. It is particularly valuable for students aiming for high grades who need experience with biological explanations, graph interpretation, calculations, practical-investigation design, experimental evaluation, and extended scientific responses under timed exam conditions.

The paper is also highly useful for Biology teachers, tutors, sixth-form colleges, revision centres, home-schooled learners, and retake candidates who require official Edexcel exam-standard resources for mock examinations, classroom assessments, practical-skills development, homework tasks, and structured revision programmes. It helps learners strengthen scientific reasoning, biological understanding, analytical skills, data interpretation, and exam technique before the 2026 AS Biology examinations.