Pearson Edexcel A Level Biology Paper 1 2025 Question Paper

Download the official Pearson Edexcel A-Level Biology A (Salters-Nuffield) Paper 1 question paper from the June 2025 examination series (9BN0/01). This examiner-authentic PDF contains the complete Paper 1 assessment used in the live Edexcel A-Level Biology examination. The paper covers The Natural Environment and Species Survival, testing advanced biological knowledge, practical understanding, data analysis, calculations, experimental design, and extended scientific explanation skills under real Edexcel exam conditions.

The paper includes questions on decomposition, forensic succession, decomposition rates, starch structure, sustainable food sources, tensile strength of plant fibres, the cell cycle, mitosis, DNA replication, stem cells, globular proteins, species definitions, gel electrophoresis, biodiversity, conservation, fluid mosaic membranes, osmosis, active transport, xylem transport, non-specific immunity, antibiotics, peptidoglycan structure, fertilisation, blastocyst differentiation, natural selection, immunotherapy, photosynthesis, carbon sinks, climate change, seed germination, enzyme activity, and Q10 investigations.

Students are required to analyse graphs, interpret experimental data, complete calculations, evaluate biological investigations, explain practical methods, and apply scientific reasoning to realistic biological scenarios involving cheetah conservation, cancer treatment, alpine plant germination, forest carbon storage, and global climate change.

The paper contains multiple-choice questions, short-answer responses, graph-analysis tasks, calculation questions, experimental-design exercises, and extended-response essays. Topics include monoclonal antibodies, checkpoint blockers, mRNA vaccines, cellulose and peptidoglycan structure, stem-cell differentiation, adaptation, ecological succession, carbon cycling, and enzyme-controlled reactions in germinating seeds.

This 100-mark assessment is ideal for timed practice, classroom revision, mock examinations, independent study, and advanced A-Level Biology preparation before the 2026 examination series. Pair this paper with the matching Edexcel 9BN0/01 June 2025 mark scheme and examiner guidance on markscheme.net for complete examiner-standard revision and accurate self-assessment.

This document is designed for Pearson Edexcel A-Level Biology A (Salters-Nuffield) students studying the 9BN0 specification, especially Year 13 students preparing for the 2026 examination series who want authentic practice using official Edexcel assessment materials. It is particularly valuable for students aiming for high grades who need experience with biological explanations, practical evaluation, graph interpretation, data analysis, calculations, and extended scientific responses under timed exam conditions.

The paper is also highly useful for Biology teachers, tutors, sixth-form colleges, revision centres, home-schooled learners, and retake candidates who require official Edexcel exam-standard resources for mock examinations, classroom assessments, homework tasks, practical-skills development, and structured revision programmes. It helps learners strengthen scientific reasoning, biological understanding, experimental evaluation, application skills, and exam technique before the 2026 A-Level Biology examinations.