Pearson Edexcel As Biology Paper 1 2025 Question Paper

Download the official Pearson Edexcel AS Level Biology A (Salters-Nuffield) Paper 1 question paper from the June 2025 examination series (8BN0/01). This examiner-authentic PDF contains the complete Paper 1 assessment used in the live Edexcel AS Biology examination. The paper covers key topics from Lifestyle, Transport, Genes and Health, testing biological knowledge, data analysis, calculations, practical understanding, and extended scientific explanation skills under real Edexcel exam conditions.

The paper includes questions on DNA and RNA structure, transcription and replication, phospholipid membranes, active transport, facilitated diffusion, inheritance patterns in chickens, genetic diagrams, and chi-squared testing. Students are required to apply biological terminology, analyse diagrams, complete calculations, and explain biological processes using scientific reasoning and accurate technical language.

Further sections assess understanding of vitamin C in broccoli, experimental design, cardiovascular physiology, angioplasty and stents, anticoagulant action, collagen structure, enzyme inhibition, osteoarthritis, Daphnia heart-rate investigations, gas exchange, emphysema, haemoglobin adaptation at high altitude, and oxygen transport. The paper also includes practical-investigation questions involving variables, controls, validity, reliability, and data interpretation based on realistic biological experiments.

The examination features multiple-choice questions, short-answer responses, calculation tasks, graph interpretation, practical-method design, and extended-response questions linked to human health, transport systems, proteins, enzymes, and respiration. Students must demonstrate knowledge of biological concepts while interpreting scientific evidence and experimental data.

This 80-mark assessment is ideal for timed practice, classroom revision, mock examinations, independent study, and advanced AS Biology preparation before the 2026 examination series. Pair this paper with the matching Edexcel 8BN0/01 June 2025 mark scheme and examiner guidance on markscheme.net for complete examiner-standard revision and accurate self-assessment.

This document is designed for Pearson Edexcel AS Level Biology A (Salters-Nuffield) students studying the 8BN0 specification, especially Year 12 students preparing for the 2026 examination series who want authentic practice using official Edexcel assessment materials. It is particularly valuable for students aiming for high grades who need experience with biological calculations, experimental design, graph interpretation, practical-analysis questions, and extended scientific explanations under timed exam conditions.

The paper is also highly useful for Biology teachers, tutors, sixth-form colleges, revision centres, home-schooled learners, and retake candidates who require official Edexcel exam-standard resources for mock examinations, classroom assessments, homework tasks, practical-skills development, and structured revision programmes. It helps learners strengthen biological understanding, scientific vocabulary, data interpretation, evaluation skills, and exam technique before the 2026 AS Biology examinations.