Pearson Edexcel A Level Biology B Paper 1 2025 Qp

Download the official Pearson Edexcel A-Level Biology B Paper 1 question paper from the June 2025 examination series (9BI0/01). This examiner-authentic PDF contains the complete Paper 1 assessment used in the live Edexcel A-Level Biology B examination. The paper covers Advanced Biochemistry, Microbiology and Genetics, testing biological knowledge, calculations, graph interpretation, experimental design, data analysis, and extended scientific reasoning under real Edexcel exam conditions.

The paper includes questions on active transport, membrane permeability, glycolysis, the Krebs cycle, ATP efficiency calculations, virus culturing and filtration, phospholipid bilayers, cholesterol and membrane fluidity, Turner’s syndrome, autoimmune disorders, antibodies, lymphocytes, photosynthesis in Chlorella, chloroplast membranes, ATP synthase, gas exchange systems, alveoli, sponges, tadpole-to-frog respiration changes, nitrogen effects on photosynthesis, RUBISCO activity, gene expression, aptamers, RT-PCR, and influenza haemagglutinins.

Students are required to analyse biological graphs, interpret scientific investigations, complete calculations, explain molecular and physiological processes, evaluate evidence, and apply biological concepts to realistic scientific contexts. Practical and analytical questions include culturing viruses, membrane-transport analysis, Chlorella growth investigations, gas-exchange calculations, photosynthetic-pigment absorption studies, and gene-expression analysis under varying nitrogen conditions.

The examination also contains extended-response discussion questions on how nitrogen levels affect photosynthesis and the advantages and disadvantages of using aptamers to prevent influenza infection. Students must apply knowledge of biochemistry, genetics, microbiology, molecular biology, respiration, photosynthesis, immunology, and gene technology to unfamiliar biological contexts.

This 90-mark assessment features multiple-choice questions, short-answer responses, calculations, practical-investigation analysis, graph interpretation, and extended synoptic essays integrating concepts from across the A-Level Biology B specification. It is ideal for timed practice, classroom revision, mock examinations, independent study, and advanced A-Level Biology preparation before the 2026 examination series.

Pair this paper with the matching Edexcel 9BI0/01 June 2025 mark scheme and examiner guidance on markscheme.net for complete examiner-standard revision and accurate self-assessment.

This document is designed for Pearson Edexcel A-Level Biology B students studying the 9BI0 specification, especially Year 13 students preparing for the 2026 examination series who want authentic practice using official Edexcel assessment materials. It is particularly valuable for students aiming for high grades who need experience with graph interpretation, calculations, practical-investigation analysis, scientific reasoning, data interpretation, and extended biological explanations under timed exam conditions.

The paper is also highly useful for Biology teachers, tutors, sixth-form colleges, revision centres, home-schooled learners, and retake candidates who require official Edexcel exam-standard resources for mock examinations, classroom assessments, practical-skills development, homework tasks, and structured revision programmes. It helps learners strengthen analytical reasoning, biological application, scientific communication, synoptic understanding, and exam technique before the 2026 A-Level Biology examinations.