Download the official Pearson Edexcel A-Level Biology B Paper 2 question paper from the June 2025 examination series (9BI0/02). This examiner-authentic PDF contains the complete Paper 2 assessment used in the live Edexcel A-Level Biology B examination. The paper covers Advanced Physiology, Evolution and Ecology, testing biological knowledge, calculations, graph interpretation, experimental design, data analysis, and extended scientific reasoning under real Edexcel exam conditions.
The paper includes questions on liver-cell organelles, hepatitis B DNA replication, lamivudine and altered nucleotides, oogenesis, meiosis, spermatogenesis, IVF embryo development, ECG traces, myogenic stimulation, anti-anxiety drugs, stroke volume calculations, sickle cell anaemia, oxygen dissociation curves, auxin effects on plant growth, rat classification, Hardy–Weinberg calculations, warfarin resistance, inulin filtration, kidney ultrafiltration, net primary productivity, Spearman’s rank correlation, rewilding strategies, retina structure, rhodopsin, rods and cones, and nocturnal versus diurnal vision adaptations.
Students are required to analyse graphs, interpret biological investigations, complete calculations, explain physiological and ecological processes, evaluate evidence, and apply biological concepts to unfamiliar scientific contexts. Practical and analytical questions include IVF fertilisation-rate analysis, cardiovascular investigations, auxin concentration experiments, rat-population genetics, kidney-filtration calculations, ecological correlation analysis, and retinal adaptation studies.
The examination also contains extended-response discussion questions on rewilding versus controlled heather burning and the adaptation of mammalian retinas to different activity patterns. Students must apply knowledge of physiology, genetics, evolution, ecology, plant biology, homeostasis, inheritance, biodiversity, and sensory biology to realistic biological scenarios.
This 90-mark assessment features multiple-choice questions, short-answer responses, calculations, practical-investigation analysis, graph interpretation, and extended synoptic essays integrating concepts from across the A-Level Biology B specification. It is ideal for timed practice, classroom revision, mock examinations, independent study, and advanced A-Level Biology preparation before the 2026 examination series.
Pair this paper with the matching Edexcel 9BI0/02 June 2025 mark scheme and examiner guidance on markscheme.net for complete examiner-standard revision and accurate self-assessment.
This document is designed for Pearson Edexcel A-Level Biology B students studying the 9BI0 specification, especially Year 13 students preparing for the 2026 examination series who want authentic practice using official Edexcel assessment materials. It is particularly valuable for students aiming for high grades who need experience with graph interpretation, statistical calculations, practical-investigation analysis, scientific reasoning, synoptic application, and extended biological explanations under timed exam conditions.
The paper is also highly useful for Biology teachers, tutors, sixth-form colleges, revision centres, home-schooled learners, and retake candidates who require official Edexcel exam-standard resources for mock examinations, classroom assessments, practical-skills development, homework tasks, and structured revision programmes. It helps learners strengthen analytical reasoning, physiological understanding, ecological application, scientific communication, and exam technique before the 2026 A-Level Biology examinations.
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