Pearson Edexcel A Level Biology Paper 2 2025 Question Paper

Download the official Pearson Edexcel A-Level Biology A (Salters-Nuffield) Paper 2 question paper from the June 2025 examination series (9BN0/02). This examiner-authentic PDF contains the complete Paper 2 assessment used in the live Edexcel A-Level Biology examination. The paper covers Energy, Exercise and Coordination, testing advanced biological knowledge, calculations, practical understanding, graph interpretation, experimental design, and extended scientific explanation skills under real Edexcel exam conditions.

The paper includes questions on pancreatic beta cells and acinar cells, mitosis, rough endoplasmic reticulum, glycoprotein processing, spirometer traces, respiratory minute ventilation, oxygen uptake during exercise, membrane structure, cholesterol positioning in phospholipid bilayers, active transport, ion uptake, photoreceptors, pupil reflexes, nerve pathways, and fovea calculations. Students are required to analyse diagrams, interpret graphs, complete biological calculations, explain physiological processes, and apply scientific reasoning to realistic biological contexts.

Further sections assess understanding of deletion mutations, transcription, hydrogen bonding in DNA, myostatin mutations in Belgian Blue cattle, mitosis, antagonistic muscles, muscle contraction, reflex-response investigations, mitochondria, aerobic respiration, proton pumps, ATP production, phytochromes, auxin, Arabidopsis growth responses, stem-cell differentiation, gene technology, restriction enzymes, ligases, coronary arteries, angina, atheroma formation, and Daphnia heart-rate investigations.

The examination features multiple-choice questions, short-answer responses, graph-analysis tasks, practical-investigation questions, calculation exercises, and extended-response essays linked to exercise physiology, respiration, coordination, gene expression, plant responses, and cardiovascular biology. Students must demonstrate knowledge of biological concepts while evaluating scientific evidence and experimental data.

This 100-mark assessment is ideal for timed practice, classroom revision, mock examinations, independent study, and advanced A-Level Biology preparation before the 2026 examination series. Pair this paper with the matching Edexcel 9BN0/02 June 2025 mark scheme and examiner guidance on markscheme.net for complete examiner-standard revision and accurate self-assessment.

This document is designed for Pearson Edexcel A-Level Biology A (Salters-Nuffield) students studying the 9BN0 specification, especially Year 13 students preparing for the 2026 examination series who want authentic practice using official Edexcel assessment materials. It is particularly valuable for students aiming for high grades who need experience with biological explanations, graph interpretation, calculations, practical evaluation, experimental design, and extended scientific responses under timed exam conditions.

The paper is also highly useful for Biology teachers, tutors, sixth-form colleges, revision centres, home-schooled learners, and retake candidates who require official Edexcel exam-standard resources for mock examinations, classroom assessments, practical-skills development, homework tasks, and structured revision programmes. It helps learners strengthen scientific reasoning, biological understanding, analytical skills, application of knowledge, and exam technique before the 2026 A-Level Biology examinations.