AQA As Biology Paper 2 2025 Question Paper Pdf

Get the official AQA AS Level Biology Paper 2 question paper from the June 2025 series (7401/2), sat on Thursday 22 May 2025 (morning). This is the exact examiner-set paper used in the live exam, featuring structured questions, calculations, data analysis, and extended-response tasks across key AS Biology topics.

The paper assesses a wide range of core content including transport systems, cell structure, biological molecules, enzymes, plant physiology, immunity, and genetics. For example, early questions include circulatory system pathways and haemoglobin adaptations (Figures 1 and 2, pages 2–4), while Question 2 focuses on cell ultrastructure and organelles such as mitochondria and the Golgi apparatus (Figure 3, page 6).

Data analysis and practical skills are heavily tested. For instance, Figure 4 (page 8) shows glucose uptake rates with and without oxygen, requiring interpretation of active transport and diffusion processes. Later sections include biochemical testing (lipid emulsion test, page 10), evaluation of cholesterol-lowering drugs using statistical data (Figure 6, page 12), and plant transpiration investigations including stomatal density calculations (Figure 7, page 14).

The paper also includes applied biology topics such as enzyme activity experiments using catalase and hydrogen peroxide (pages 20–23), bacterial structure and disease transmission (Figure 11, page 24), and immune system responses to HIV infection (Table 4, page 28). Extended-response questions cover meiosis, genetic variation, and tissue fluid formation (pages 30–31), testing high-level understanding and exam technique.

This paper is ideal for realistic exam practice, helping you master calculations, interpret biological data, and structure high-mark answers. Pair it with the official mark scheme for full examiner-standard revision. Fully updated for the 2025/2026 academic year and optimised for instant download on markscheme.net.

This paper is essential for Year 12 students preparing for AQA AS Level Biology exams and Year 13 students consolidating core A-Level Biology knowledge. It is also ideal for retake candidates and independent learners who need authentic exam-style practice.

Teachers and tutors use this paper for mock exams, timed assessments, and structured revision sessions. It is particularly useful for developing data interpretation, calculation accuracy, and extended-response writing skills required for high-mark Biology answers.
The paper assesses a wide range of core content including transport systems, cell structure, biological molecules, enzymes, plant physiology, immunity, and genetics. For example, early questions include circulatory system pathways and haemoglobin adaptations (Figures 1 and 2, pages 2–4), while Question 2 focuses on cell ultrastructure and organelles such as mitochondria and the Golgi apparatus (Figure 3, page 6).

Data analysis and practical skills are heavily tested. For instance, Figure 4 (page 8) shows glucose uptake rates with and without oxygen, requiring interpretation of active transport and diffusion processes. Later sections include biochemical testing (lipid emulsion test, page 10), evaluation of cholesterol-lowering drugs using statistical data (Figure 6, page 12), and plant transpiration investigations including stomatal density calculations (Figure 7, page 14).

The paper also includes applied biology topics such as enzyme activity experiments using catalase and hydrogen peroxide (pages 20–23), bacterial structure and disease transmission (Figure 11, page 24), and immune system responses to HIV infection (Table 4, page 28). Extended-response questions cover meiosis, genetic variation, and tissue fluid formation (pages 30–31), testing high-level understanding and exam technique.

This paper is ideal for realistic exam practice, helping you master calculations, interpret biological data, and structure high-mark answers. Pair it with the official mark scheme for full examiner-standard revision. Fully updated for the 2025/2026 academic year and optimised for instant download on markscheme.net.