AQA As Biology Paper 1 2025 Question Paper Pdf

Get the official AQA AS Level Biology Paper 1 question paper from the May 2025 series (7401/1), sat on Monday 12 May 2025 (morning). This is the exact examiner-set paper used in the live exam, featuring structured questions, calculations, and data analysis across core AS Biology topics.

The paper covers key AQA 7401 topics including biological molecules, enzymes, nucleic acids, ATP, cell membranes, transport mechanisms, genetics, biodiversity, and gas exchange. For example, early questions test amino acids and peptide bond formation (pages 2–3), while Question 2 explores ATP structure and energy transfer (page 6). DNA replication is assessed in detail (Question 3, page 8), including experimental evidence using centrifugation results shown in Figure 5 (page 9).

Later sections include genetic code and mutations (Question 4, pages 10–11), biodiversity and measurement uncertainty (Question 5, pages 12–13), and lung function analysis using graphical data (Question 6, pages 14–16). Practical skills are also tested, such as interpreting beetroot membrane permeability experiments (Question 8, pages 20–23) and evaluating real biological data.

This paper is ideal for realistic exam practice, helping you develop data interpretation, calculation accuracy, and structured written responses required for AS Biology success. Pair it with the official mark scheme for full examiner-standard revision. Fully updated for the 2025/2026 academic year and optimised for instant download on markscheme.net.

This paper is essential for Year 12 students preparing for AQA AS Level Biology exams, as well as Year 13 students revising core foundational topics for A-Level Biology. It is also ideal for retake students and independent learners who need authentic exam-style practice.

Teachers and tutors use this paper for mock exams, class assessments, and structured revision sessions. It is particularly useful for developing data analysis, calculation skills, and understanding of key AS Biology concepts required for progression to full A-Level study.