Get the official AQA GCSE Combined Science: Trilogy Biology Paper 2 Higher Tier question paper from the June 2025 exam series (8464/B/2H). This examiner-authentic PDF contains the complete Higher Tier Biology Paper 2 in its original AQA exam layout, including genetics calculations, Punnett-square inheritance questions, quadrat sampling investigations, biodiversity analysis, evolution and fossil questions, contraception and hormone explanations, diabetes data interpretation, and six-mark extended-response biology questions exactly as candidates received them in the live examination.
This 2025 Biology 2H paper assesses inheritance, chromosomes, genes, alleles, reproduction hormones, contraception methods, ecology fieldwork, quadrat and point-quadrat sampling, GM crops, biodiversity, pollination, peat decay and climate change, evolution, fossils, classification, binomial naming, insulin and glucagon, Type 1 and Type 2 diabetes, and C-peptide analysis across the AQA GCSE Combined Science specification. Pair it with the matching AQA 8464/B/2H mark scheme on markscheme.net for complete examiner-standard revision, self-marking, and timed mock-exam practice. Updated for the 2025/2026 academic year, optimised for mobile and desktop, and instantly downloadable from markscheme.net.
This document is designed for GCSE Combined Science: Trilogy Higher Tier students preparing for the 2026 AQA examination series, including Year 11 candidates targeting grades 7–9, resit students improving previous GCSE Biology results, and home-educated learners following the AQA 8464 specification. It is especially valuable for practising Higher Tier genetics calculations, ecology data analysis, graph interpretation, evaluation questions, and six-mark extended responses under authentic exam conditions.
It is also essential for biology teachers, science departments, private tutors, revision-course providers, and parents supporting GCSE revision at home. The paper can be used for classroom mocks, intervention sessions, retrieval-practice tasks, structured revision programmes, and examiner-style assessment using the official June 2025 mark scheme.
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